Estradiol optimal range and reference range

Compiled by Alina Radu, founder of Sera, from the published sources below · Last reviewed November 9, 2026

Sera's bands for Estradiol, which differ for men and women:

Female: Optimal 50 – 200 pg/mL (184 – 734 pmol/L); sufficient 15 – 350 pg/mL (55 – 1,287 pmol/L); out of range below 15 or above 350 pg/mL.
Male: Optimal 20 – 40 pg/mL (73 – 147 pmol/L); sufficient 10 – 40 pg/mL (37 – 147 pmol/L); out of range below 10 or above 40 pg/mL.

Evidence grade: Published reference interval. A band that primarily reflects a published adult laboratory or population reference interval. It is not necessarily applicable to every assay or laboratory.

All units

Unit	Applies to	Optimal	Sufficient	Out of range
pg/mL	Female	50 – 200	15 – 350	below 15 or above 350
pg/mL	Male	20 – 40	10 – 40	below 10 or above 40
pmol/L	Female	184 – 734	55 – 1,287	below 55 or above 1,287
pmol/L	Male	73 – 147	37 – 147	below 37 or above 147
ng/L	Female	50 – 200	15 – 350	below 15 or above 350
ng/L	Male	20 – 40	10 – 40	below 10 or above 40

What the evidence says

The bands are laboratory reference intervals (Mayo Clinic Laboratories, mass spectrometry): 10 to 40 pg/mL in men and 15 to 350 pg/mL in premenopausal women across the whole cycle. No optimal band is published for either sex; the ones shown are functional targets. In women estradiol swings widely: low early in the follicular phase (roughly 12 to 166 pg/mL), peaking just before ovulation (up to about 500), with a second mid-luteal rise, so a single value only means something alongside cycle day. Levels fall sharply after menopause, typically below 10 pg/mL. These ranges are informative, not diagnostic.

For women: These ranges are informative, not diagnostic, and reflect a follicular-phase reference for women in their reproductive years (pre-menopausal). Estradiol swings dramatically across the menstrual cycle — low early in the follicular phase, peaking just before ovulation, with a second mid-luteal rise — so a single value is only meaningful alongside your cycle day. Levels fall sharply after menopause.

Menstrual cycle: Female range is a follicular-phase reference.

Assay: Results differ meaningfully between platforms and methods. Compare against the interval printed by the issuing laboratory before acting on a borderline value.

Sources

Estradiol, Serum (EEST) — Mayo Clinic Laboratories, 2026 · Laboratory / reference-interval sourceAdults by mass spectrometry: men 10 to 40 pg/mL, premenopausal women 15 to 350 pg/mL (varies widely through the cycle), postmenopausal women below 10. Conversion: pg/mL x 3.676 = pmol/L.
Estradiol (test 004515) — Labcorp, 2026 · Laboratory / reference-interval sourceAdult men 7.6 to 42.6 pg/mL. Women: follicular 12.5 to 166.0, ovulation 85.8 to 498.0, luteal 43.8 to 211.0, postmenopausal below 6.0 to 54.7. Roche immunoassay. Confirms a male upper limit near 40 and shows how wide the female range is by phase.

What Estradiol (E2) measures

The main form of estrogen.

Estradiol affects bone strength, heart health, mood, and the reproductive system. Levels rise and fall across the menstrual cycle.

Related hormone health markers

See Estradiol in the full list of biomarker ranges

This page is a reference for practitioners, not medical advice. Sera's bands are practitioner targets for structured review, not diagnostic thresholds, and Sera does not diagnose or recommend treatment. Read a result alongside the interval printed by the issuing laboratory and the client's history and symptoms. Range set: Sera Production, version 1.0, last reviewed November 9, 2026.